Figure 4

sds22 mutant cells accumulate G-actin and exhibit breakdown of F-actin. sds22 mutant clones in ovarian follicle cells and giant nurse cells, marked by the absence of GFP. (A) Globular actin (G-actin, red) is upregulated in sds22 mutant clones in follicle cells. (B) Globular actin (G-actin, red) is also upregulated in sds22 mutant nurse cells. (C) Nurse cell membranes, lined with filamentous actin (marked by Phalloidin, red), break down in sds22 mutant nurse cells, leading to large cells containing multiple nuclei.