Figure 1

sds22 is required to maintain epithelial morphology in imaginal discs. Mitotic recombination clones were induced in developing wing imaginal discs with the hs.FLP system and marked by the absence of GFP. (A) Wildtype clones induced 84 hrs after egg laying (AEL) and allowed to grow for 2 days. (B) sds22 mutant clones induced 84 hrs AEL grow to a similar size as wildtype clones after 2 days. (C) sds22 mutant clones induced 60 hrs AEL are largely eliminated after 3 days. (D) Wildtype clones induced in a Minute background at 84 hrs AEL grow to large sizes after 2 days. DAPI marks nuclei. (E) sds22 mutant clones in a Minute background induced at the same developmental stage (84 hrs AEL) grow to smaller sizes and exhibit morphological defects after 2 days. DAPI marks nuclei. (F) sds22 mutant clones induced earlier in development (60 hrs AEL) and allowed to grow for 3 days are eliminated and often leave ectopic folds behind. DAPI marks nuclei. (G) sds22 mutant clones induced at 84 hrs AEL and allowed to grow for 2 days. The morphogenesis defect in mutant clones was visualised by staining for phalloidin and DAPI. The arrow points to an island of wild-type tissue surrounded by deeply folded sds22 mutant tissue. (H) Phalloidin channel of (G). (I) DAPI channel of (G).