Neuroblastic and glial immunophenotype in NB cell line subtypes. Neurofilament NF68 cytoplasm expression intensity was higher in N-, I- than in S-type cell lines. Vimentin is expressed intensely in the cytoplasm of the three cell lines subtypes. GD2 cell membrane and intense nuclear membrane calcyclin staining allows to distinguish between the neuroblastic and the glial phenotype. Blue: DAPI, Green: FITC.