Figure 6
From: Generation of diverse neuronal subtypes in cloned populations of stem-like cells
Non-differentiated, SSEA-1 positive cells re-cloned from differentiated NE-4C cultures display neural stem cell properties. Non-differentiated cells re-cloned from differentiated (stage 6) cultures displayed epithelial morphology (A) but gave rise to neurons (B) and astrocytes (C) if induced with RA. βIII-tubulin immunoreactive neurons (B) and GFAP-positive astroglial cells (C) visualized by HRP-DAB reaction are shown in a representative clone (clone 0901). Bars: 50 μm on A, C and 100 μm on B. D: Investigated sub-clones (clone 0901; 0905; 1204) showed a gene expression pattern characteristic to non-induced (stage1) NE-4C cells, but expressed a variety of region-specific genes after reaching more differentiated stage (st4).