Dumpy-30 family members as determinants of male fertility and interaction partners of metal-responsive transcription factor 1 (MTF-1) in Drosophila

Table 1 Sperm presence and motility in female reproductive tract

Male genotype	Female genotype	Days after cross	Number of tested females	Number of females with sperm in seminal receptacle		Number of females with sperm in uterus/oviduct
				Sperm	Motile	Sperm	Motile
Oregon R	Oregon R	5	6	5	5	1	No
	XY hs-tra*	5	6	4	4	4	1
	XY hs-tra*	1	6	6	6	4	4
dpy-30L2^6-1	Oregon R	5	6	0	-	0	-
	XY hs-tra*	5	10	0	-	3	No
	XY hs-tra*	1	6	0	-	6	No

XY hs-tra* – XY fly carrying a single copy of the hs-tra-female plasmid [54]. These flies are females, which contain no eggs, but rather small nonfunctional germ cells in their gonads. As no eggs are laid, sperm can be detected in the uterus or in oviducts long after mating. The experiment shows that mutant sperm is not stored in the seminal receptacle and that it is immotile when remaining in the uterus or in the oviduct. In contrast, motile wildtype sperm is found both in seminal receptacles and in the uterus or oviducts of the analyzed females.

ISSN: 1471-213X