Figure 4

Reduced DNA binding of dMTF-1 upon co-expression of Dpy-30L1. DNA binding by dMTF-1 was determined by EMSA. Drosophila S2 cells were transfected with VSV tagged dMTF-1 expression plasmids, and 20 μg of nuclear protein extract was used for each bandshift reaction. Lanes 1 and 4, bandshift with [³²P]-labeled MRE consensus oligonucleotide (MRE-s). Lanes 2 and 5, same conditions but also including a 200-fold excess of unlabeled MRE-s competitor oligonucleotide (specific competitor). Lanes 3 and 6, same conditions but with a 200-fold excess of unlabeled Sp1 oligonucleotide (nonspecific competitor). Cells had been treated for 6 hours with medium containing 100 μM zinc sulfate.