Figure 3

Inhibitory effect of Dpy-30L1, Dpy-30L2 and their mammalian orthologs is restricted to Drosophila MTF-1. The ratio of firefly to renilla luciferase activity in transiently transfected Drosophila Schneider S2 cells is shown. Reporter, MtnA promoter fused to firefly luciferase; reference, tubulin promoter fused to renilla luciferase. Dpy-30L1 and the mouse ortholog were under the control of the actin promoter. 72 hours after transfection, the medium in half of the plates was supplemented with 40 μM cadmium chloride for 24 hours, while the others served as controls. A) Mouse MTF-1 was co-transfected in the indicated samples; B) Drosophila MTF-1 was co-transfected in the indicated samples.