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Figure 7 | BMC Developmental Biology

Figure 7

From: Endothelial-specific ablation of Serum Response Factor causes hemorrhaging, yolk sac vascular failure, and embryonic lethality

Figure 7

SRF protein is decreased in endothelial tissues of Tie2Cre+/-Srff/fembryos. Photomicrographs (A, C:1,000×; B, D:4,000×) of cross-sections of blood vessels from E10.5 wild-type (A, B) and mutant (C, D) embryos were used to count the total number of VEC and the number of VEC exhibiting SRF (boxes in A, C correspond to images in C, D respectively). Tissues were labelled with antibodies against SRF (green) and the VEC-marker PECAM-1 (red) and analyzed using single-plane confocal laser microscopy (blue = Topro3 nuclear staining). Only those cells showing SRF signal completely encircled by PECAM-1 staining were scored as SRF-positive VEC (B, D arrowheads). Not all VEC displayed detectable SRF protein (A, arrow). Final numbers were expressed as percent SRF-positive VEC of the total counted for that vessel (E): 84 ± 14.3 vs. 25 ± 15.7, wild-type vs. mutant respectively; *p = 0.0000004, student's t test. Scale bars: A, C = 30 μm; B, D = 10 μm.

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