Figure 8
From: Yolk protein is expressed in the insect testis and interacts with sperm
YP2 protein forms transient extracellular coat on spermatozoa. (A) Western blot detected YP2 immunoreactivity in both the UVD seminal fluid (Lane 1) and the UVD sperm (Lane 2). After sperm fraction was treated with 1 M NaCl, YP2 signal was absent from sperm (Lane 3) but detected in the wash (Lane 4). (B-C) YP2 immunoreactivity was detected on both nucleated (B) and anucleated (C) spermatozoa from the UVD lumen. (D-E) The staining was absent in both types of spermatozoa from the seminal vesicles. Thread-like sperm nuclei aligned in nucleated bundle in (B) and (D) are stained blue with Hoechst 33258. Bar = 20 μm.