Figure 7
From: Yolk protein is expressed in the insect testis and interacts with sperm
YP2 is produced in the cyst cells. (A) Total RNA was extracted separately from ovaries, testicular sperm bundles, UVD, and male fat body. RT-PCR was performed using yp2-specific primers; 28S r-RNA gene was amplified as RNA loading control. A 660 nt PCR product representing yp2 was obtained from ovary (Ov, control) and from cyst cell-enclosed sperm bundles (CC) but not from the UVD epithelial cells or fat body (FB). M – markers. (B) No YP2 staining was observed in the UVD epithelium after the ligature was applied between testis and the UVD. (C) YP2 protein was detected in the UVDs of control sham-operated males. In all preparations nuclei were stained blue with Hoechst 33258. Bar = 20 μm.