Figure 1

dAux mutations cause defects in photoreceptor specification. (A) A schematic diagram of Drosophila Auxilin, consisting of a N-terminal kinase domain (orange), a PTEN-related domain (blue), clathrin binding motifs (CBM, yellow), and a J-domain (pink). The molecular lesions in eight dAux alleles are shown, and those isolated with a FRT chromosome are indicated in blue. (B-E) Fluorescent micrographs of larval eye discs from (B, C) Act5C>FLP/+; FRT^5-5Z3515, dAux^L78H/FRT^5-5Z3515, ubi-GFP^nlsand (D, E) Act5C>FLP/+; FRT^5-5Z3515, dAux^F956*/FRT^5-5Z3515, ubi-GFP^nlsanimals. The mosaic discs are stained for Elav (red), which labels the nuclei of neuronal cells, and mutant tissues are marked by the absence of nuclear-GFP expression (green). (C, E) Boxed areas of dAux^L78Hand dAux^F956* clones from B and D are shown at a higher magnification. In all the panels, anterior is to the left. Scale bar, 50 μm.