Figure 4

Morphological changes in midgut morphology during development. Panels A-F are from larvae of 5th-HCS, 6th-24 h (feeding), 6th-96 h (wandering), p-1 d (pupa 24 h after ecdysis), p-6 d (pupa 6 d) and preadult (p-9 d). Mg, midgut; Fb, fat body; Hg, Hindgut. Panels G-I, cryosections of midguts from molting 5th instar (5th-HCS), feeding 6th instar (6th-24 h) and 6th-96 h larvae stained with DAPI. Panels J-L, location of red spots. J) Midgut from 6th-96 h larvae (surface, UV and white light). K) Red larval midgut and the imaginal cells of the 6th-96 h midgut (dissected, UV and white light). L) Cryosection of 6th-96 h midgut observed under UV light. L-1 and L-2) Midguts from 6th-96 h larva and p-5 d pupa and observed directly under white light without other treatment. Panels M-O) DAPI in whole mount midguts from 6th-48 h, 6th-60 h, and 6th-96 h larvae, showing formation of the imaginal cell layers. M-1 and N-1) white light images. O-1) Merged white and UV light images. lac, larval midgut; imc, imaginal cells; Lu, lumen of midgut; red spot.