Validation of reference genes for quantitative RT-PCR studies in porcine oocytes and preimplantation embryos

Table 3 Ranking of genes by expression stability.

All genes included	Minus GAPDH	Minus PGK1	Minus H2A	Minus Blastocysts
1: GAPDH	2: UBC	2: UBC	1: GAPDH	1: GAPDH
2: UBC	3:PGK1	1: *GAPDH*	3:PGK1	3: *PGK1*
3: PGK1	4:S18	4:S18	4:S18	2: *UBC*
4: S18	5:H2A	5:H2A	2: *UBC*	4:S18
5: H2A	6: BACT	6: BACT	6: BACT	5:H2A
6: BACT	7:B2M	7:B2M	7:B2M	7: *B2M*
7: B2M	X	X	X	6: *BACT*

In the first column, all genes and developmental stages were included in the analysis, and in the following columns the genes GAPDH, PGK1, or H2A were excluded, and in the last column, blastocyst stage embryos were excluded. The numbers represent ranking position of that gene when all genes are included. In bold are those genes from which the ranking is affected by the exclusion.

ISSN: 1471-213X