Figure 6

Long-term YFP expression by Cre transfected NPCs in vivo after transplantation into adult mouse brains. A-C: At 7-days-post-transplantation, cells did not migrate and remained as bolus at the injection site (A). Cells co-expressed YFP and Nestin (C) but not GFAP (B). Note the strong reaction of the tissue surrounding the graft as revealed by GFAP immunostaining at this early time point. D-E: At one month post-transplantation, intense migration of YFP-positive cells from the transplantation site along the corpus callosum was observed in all animals (D). E: Projection of a confocal stack spanning 15 μm in the z-dimension, showing a typical example of a fully differentiated YFP-positive cell expressing the astrocytic marker GFAP at 1 month post-transplantation. F: 3-dimentional projection of a confocal stack spanning 25 μm in the z-dimension, showing a typical example of a fully differentiated YFP-positive cell expressing the oligodendrocyte marker NG2 (arrow) in the corpus callosum at 1 month post-transplantation. Note the presence of NG2+/YFP- and YFP+/NG2- cells (asterisks) in the same region. cc: corpus callosum