Figure 3
From: Coordinate regulation of DNA methyltransferase expression during oogenesis
Methylation profile of a) Snrpn , Peg3 , Igf2r , H19 and b,c) IAP and LINE-1 repeats in Dnmt3L -/- oocytes. a,b) Bisulfite sequencing results are depicted as follows: each line represents an individual clone; a filled circle indicates a methylated CpG site, an open circle denotes an unmethylated CpG and a missing circle represents a CpG site where the sequencing data were ambiguous. H19 methylation served as control for somatic cell contamination. While Snrpn, Peg3 and Igf2r appear to be abnormally unmethylated (a), IAP LTR methylation is unaffected in Dnmt3L homozygous oocytes at 15 dpp (b). c) Methylation sensitive Southern analysis of IAP LTR and LINE-1 5'UTR methylation in Dnmt3L +/+ and -/- cumulus cells (soma) and mature metaphase II oocytes (oo). Lanes headed by M and H contain DNA that was cleaved with MspI and HpaII, respectively. Repeat sequences appear to be normally methylated in DNMT3L depleted oocytes.