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Figure 4 | BMC Developmental Biology

Figure 4

From: Time-dependent patterning of the mesoderm and endoderm by Nodal signals in zebrafish

Figure 4

Nodal signals pattern the mesoderm during a three-hour time window. (A1-A8) Trunk somites form in embryos treated at 3.7 h (A1, 5), but flh is expressed in four ectodermal domains (A6). (B1-B8) Embryos treated at 4 h contain more somites (B5). flh is expressed at the midline and small amounts of notochord tissue are observed in live embryos (B1, arrow). pax2.1 expression is also observed (B7). At later time points, embryos have progressively more somites and more notochord (C1-E7). flh expression extends further up the midline and pax2.1 is expressed more strongly. Kupffer's vesicle forms in embryos treated 4.3 h (C3, arrowhead). shhb is not expressed in embryos treated before gastrulation (A8-E8). Images of live embryos at 24 h, anterior to the left (A1-E1; A2-E2) or 14hpf (A3-E3); dorsal views of fixed embryos at 10 h (A4-E4; A6-E6; A8-D8) or 14 h (A5-E5; A7-E7). Control embryos are depicted in Fig. 1J-S, which are from the same experiment.

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