Figure 2

mfr testis cDNA analysis reveals alternatively spliced transcripts and predicts multiple isoforms. For each cDNA shown in (A), the start and stop codons that define the longest ORFs and length of the ORF are indicated. For cDNA T1, the ATG and ORF length is deduced from additional 5'RACE and phylogenetic data as indicated by the parentheses. cDNA T1 is used as a reference to note where splicing differences give rise to alternative exons (gray). (B) shows the multiple Mfr protein isoforms predicted from testis cDNA analysis. The number of amino acids and positions of C2 (C2B-C2F) and transmembrane (TM) domains are noted for each predicted protein.