Figure 4From: Identification of the C. elegansanaphase promoting complex subunit Cdc26 by phenotypic profiling and functional rescue in yeastThe C. elegans B0511.9 gene complements the proliferation defect of yeast cells lacking the CDC26 gene. (A) S. cerevisiae cells containing a deletion of CDC26 were transformed with a plasmid expressing the C. elegans B0511.9 gene from the yeast PGK promoter, or with the empty vector, or with a plasmid containing the yeast CDC26 gene. Wild type cells (WT) transformed with vector and cells lacking the DOC1/APC10 gene transformed with plasmids containing B0511.9 or the yeast DOC1 gene served as controls. Transformants were grown at 25°C in selective medium, normalized for cell density, and tenfold serial dilutions were spotted onto plates with rich medium. Shown are plates incubated at 25°C for 36 hours and at 37°C for 24 hours. (B) Strains from (A) were grown at 25°C and then shifted to 37°C for 5 hours. Shown are differential interference contrast pictures of the cells. Numbers indicate the percentage of budded cells in the indicated cultures. Graphs show cellular DNA content measured with a flow cytometer.Back to article page