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Figure 4 | BMC Developmental Biology

Figure 4

From: Identification of the C. elegansanaphase promoting complex subunit Cdc26 by phenotypic profiling and functional rescue in yeast

Figure 4

The C. elegans B0511.9 gene complements the proliferation defect of yeast cells lacking the CDC26 gene. (A) S. cerevisiae cells containing a deletion of CDC26 were transformed with a plasmid expressing the C. elegans B0511.9 gene from the yeast PGK promoter, or with the empty vector, or with a plasmid containing the yeast CDC26 gene. Wild type cells (WT) transformed with vector and cells lacking the DOC1/APC10 gene transformed with plasmids containing B0511.9 or the yeast DOC1 gene served as controls. Transformants were grown at 25°C in selective medium, normalized for cell density, and tenfold serial dilutions were spotted onto plates with rich medium. Shown are plates incubated at 25°C for 36 hours and at 37°C for 24 hours. (B) Strains from (A) were grown at 25°C and then shifted to 37°C for 5 hours. Shown are differential interference contrast pictures of the cells. Numbers indicate the percentage of budded cells in the indicated cultures. Graphs show cellular DNA content measured with a flow cytometer.

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