Figure 1From: Identification of the C. elegansanaphase promoting complex subunit Cdc26 by phenotypic profiling and functional rescue in yeastMeiotic metaphase arrest induced by strong RNAi of B0511.9. Pairs of pictures show DIC and fluorescence images of embryos in the uterus of a mother carrying a GFP::H2B transgene marking nuclei. (A, B) wild-type embryos show progressively more nuclei as divisions proceed. (C, D) B0511.9(RNAi) embryos are all arrested at the one cell stage; staining of such embryos for beta-tubulin (red) and DNA (blue) shows arrest stage is at meiotic metaphase I (inset). (E, F) emb-27/Cdc16(RNAi) embryos arrested in metaphase of meiosis I [15], a phenotype identical to that of B0511.9(RNAi) in (C, D). Arrows in (D) and (F) point to sperm chromatin, indicating that the embryos have been fertilized.Back to article page