Figure 5

CRMP2 is a substrate for Fps and Fer but inhibits their in vivo autophosphorylation activity. HEK293T cells were co-transfected with expression plasmids encoding Myc-tagged Fps or Fer with or without Myc-tagged CRMP2. (A), The in vivo tyrosine phosphorylation status of Fps and Fer were assessed by immunoprecipitating them with anti-Myc antibody followed by immunoblotting with anti-pY antibody (upper). The blot was then stripped and reprobed with anti-Myc to reveal the level of Fps and Fer expression (lower). (B), The in vivo tyrosine phosphorylation status of CRMP2 was also assessed by anti-pY immunoblotting the anti-Myc immunoprecipitates (upper). The blot was then stripped and reprobed with anti-Myc to reveal the level of CRMP2 expression (lower).