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Figure 2 | BMC Developmental Biology

Figure 2

From: The Fer tyrosine kinase regulates an axon retraction response to Semaphorin 3A in dorsal root ganglion neurons

Figure 2

In vitro kinase activity of Fps and Fer isolated from embryonic, neonatal and adult mouse brains. Whole brains from embryonic (E18), neonatal or adult wt mice were lysed in kinase lysis buffer and protein concentrations were determined. Fps and Fer were immunoprecipitated with anti Fps/Fer antibody from equal amounts of protein. The immune precipitates were processed for immune complex kinase assay with or without added ATP as described in Materials and Methods, and tubulin was added as exogenous substrate. (A), Supernatants containing tubulin were immunoblotted with anti-pY to reveal the degree of tubulin phosphorylation (upper). The blot was then stripped and reprobed with anti-tubulin antibody (lower). (B), Immunoprecipitated Fps and Fer proteins were dissociated from the beads and analyzed by immunoblotting with anti-pY to reveal the degree of in vitro Fps/Fer autophosphorylation (upper). The blot was then stripped and reprobed with anti-Fps/Fer antibody (lower).

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