Figure 1
In vivo expression and tyrosine phosphorylation status of Fps and Fer in neonatal mouse brains and DRGs. (A) Fps and Fer were immunoprecipitated from neonatal mouse brain lysates from the indicated mouse strains (wt, fpsKR/KR, ferDR/DRor fpsKR/KR/ferDR/DR) with anti-Fps/Fer antibody and immunoblotted with anti-pY (upper panel). The blot was stripped and reprobed with anti-Fps/Fer (lower panel). (B) DRG from indicated strains (wt, fpsKR/KR, ferDR/DRor fpsKR/KR/ferDR/DR) were dissected and subjected to immunoblotting with anti-Fps/Fer antibody. The positions of Fer, Fps and a non-specific (NS) band are indicated.