Figure 6
From: The oxidative stress adaptor p66Shc is required for permanent embryo arrest in vitro
Oocyte microinjection. The short hairpin (sh)RNAi microinjection of a the germinal vesicle (GV) stage bovine oocyte. (A) The oocyte has been localized and is subsequently microinjected with shRNAi molecules (B). The oocyte is being held by the holding pipette and microinjected by a pipette of 4 μm in diameter and beveled at 35°. Care was taken not to strip off the cumulus cells surrounding the GV oocytes and to avoid perforating the germinal vesicle during microinjection.