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Figure 3 | BMC Developmental Biology

Figure 3

From: The oxidative stress adaptor p66Shc is required for permanent embryo arrest in vitro

Figure 3

p66Shc protein levels following microinjection of p66 shRNA interfering molecules. The distribution of p66Shc in early bovine embryos was assessed (A, B, C and D). Green, red and blue colors in each representative photomicrograph indicate positive staining for the respective primary antibody: p66Shc (FITC), F-actin (Rhodamine phalloidin), and nuclei (DAPI) respectively. Panels A and B represent Uninjected and Injected (Vehicle) controls respectively. Panel C (shRNAi low dose = ~12,000 molecules) and Panel D (shRNA high dose = ~24,000 molecules) display a marked reduction in p66 fluorescence. Panel E represent the no primary antibody controls. Scion Image analysis resulted in the quantification of FITC immunofluorescence (p66Shc) across different groups (F). Relative signal strengths are presented as the mean ± S.E.M. representative of three independent replicates. Bars with different letters represent significant differences in relative signal strength between treatment groups (P ≤ 0.05).

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