Figure 3From: The oxidative stress adaptor p66Shc is required for permanent embryo arrest in vitrop66Shc protein levels following microinjection of p66 shRNA interfering molecules. The distribution of p66Shc in early bovine embryos was assessed (A, B, C and D). Green, red and blue colors in each representative photomicrograph indicate positive staining for the respective primary antibody: p66Shc (FITC), F-actin (Rhodamine phalloidin), and nuclei (DAPI) respectively. Panels A and B represent Uninjected and Injected (Vehicle) controls respectively. Panel C (shRNAi low dose = ~12,000 molecules) and Panel D (shRNA high dose = ~24,000 molecules) display a marked reduction in p66 fluorescence. Panel E represent the no primary antibody controls. Scion Image analysis resulted in the quantification of FITC immunofluorescence (p66Shc) across different groups (F). Relative signal strengths are presented as the mean ± S.E.M. representative of three independent replicates. Bars with different letters represent significant differences in relative signal strength between treatment groups (P ≤ 0.05).Back to article page