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Figure 2 | BMC Developmental Biology

Figure 2

From: The oxidative stress adaptor p66Shc is required for permanent embryo arrest in vitro

Figure 2

Real Time PCR quantification of p66Shc mRNA levels at the 2-cell stage following RNAi. Real Time PCR analysis of p66Shc (A) and H2a (B) mRNA levels in 35 hpi embryos following control IVF, microinjection of the vehicle and microinjection with 12,000 p66Shc hairpin RNAi molecules. p66Shc mRNA levels decreased in p66Shc RNAi microinjected embryos (*P < 0.001), while Histone H2a mRNA levels showed no significant differences (P = 0.744) among the three groups. This result provided evidence that the down-regulation of p66Shc mRNA levels following p66Shc hairpin RNAi molecule injection is selective for p66Shc transcripts. Real Time PCR analysis of p53 mRNA levels (C) for the three groups was also performed, showing no significant differences (P = 0.98). The experiments were conducted on three different pools of 80–100 embryos (n = 3) and replicated three times (r = 3) for each sample. The data were normalized for the content in one embryo.

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