Figure 1

Schematic representation of the amplification strategy for AmphiHtt cDNA isolation. The coding sequence of AmphiHtt is represented by a horizontal line. Four clones were isolated by RT-PCR from an adult amphioxus (empty arrows), and five clones by PCR from an embryonic cDNA library (grey arrows). The numbers in brackets represent the nucleotide position in each fragment. The horizontal lines also show the start of translation (Met) and the stop codon (TGA). The clones used to synthesise the probes are shown as probe A/B/C.