Figure 5

Tyrosine phosphorylated proteins after stimulation with IGF-I. A: Protein lysates from cells treated with IGF-I for 20 mins were analysed by Western blot using a specific antibody against tyrosine phosphorylated proteins. B: To ensure equal loading used the MemCode Reversible™ protein stain (Pierce Biotechnology). C: The graph represents the change in tyrosine phosphorylation after ensuring for equal loading. The graph represents the results for FDF (n = 5; closed bar un-stimulated, diamonds represents stimulated cells) and MDF (n = 5 hash bar represents un-stimulated and the closed bar stimulated). Error bars = SEM. A statistical increase was shown in FDF following stimulation with IGF-I compared to MDF at 50 kDa (*p = 0.028); a further statistical difference was shown with an increase of a band at 66 kDa in unstimulated FDF cells compared to unstimulated MDF (**p = 0.024). Two way ANOVA showed a statistical significant (p = 0.007) difference in the mean values among the different cell type greater than would be expected by chance allowing for the difference in treatment.