Table 1 Primers used for RT-PCR experiments.
From: Investigation of MYST4 histone acetyltransferase and its involvement in mammalian gametogenesis
Gene | Primer sequences | Accession numbers | Length of PCR products (bp) | TM(°C) |
|---|---|---|---|---|
MYST4 (complete sequence) | Up 5'-ATGTTCTTTCACCCGAATGC-3' | AF119231 | 6590 | 55 |
| Â | Low 5'-AAATAAATGGTGCCAACAAATG-3' | NM_017479 | Â | Â |
MYST4 | Up 5'-AGTTCACAAAAGTCCAGCAC-3' | AF119231 | 300 | 60 |
| Â | Low 5'-GCTAGAGGAGGAGGAGTGAG-3' | XM_880782 | Â | Â |
all-MYST4-iso | Up 5'-ACCTTCAGCCTTGCCAAACTG-3' | AF119231 | 296 | 60 |
| Â | Low 5'-TCTTTGGCTGTGAGAGATGC-3' | Â | Â | Â |
TUBULIN | Up 5'-CTCTGCTGAGAAAGCCTACCA-3' | BC018948 | 400 | 60 |
| Â | Low 5'-CCACGTACCAGTGAACAAAGG-3' | Â | Â | Â |