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Figure 6 | BMC Developmental Biology

Figure 6

From: The Drosophila Perlecan gene trol regulates multiple signaling pathways in different developmental contexts

Figure 6

Dpp, Wg and Hh signaling are affected in trol mutants in second instar brains but not in third instar brain lobes/eye discs. qRT-PCR analysis of A) the expression levels of dpp, its response gene salm, wg and its response gene slp in the ventral ganglia of second instar larvae. Data for the Hh response gene ptc is shown in Fig. 3. B) Expression levels of dpp, its response gene salm, wg and its response gene slp, hh and its response gene ptc in trolsdmutant third instar brain lobes/eye discs. C) Expression of dpp and salm in hsGAL4/+; +/(EP)dad second instar ventral ganglia and hsGAL4 controls. D) Expression of wg and slp in hsGAL4/+; +/(EP)sgg second instar ventral ganglia and hsGAL4 controls. In all panels, error bars indicate standard error. All reactions were carried out in triplicate at three different template concentrations to ensure amplification was in the linear range. β-actin was used as an internal normalization control.

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