Figure 3

Hh and Bnl signaling activity in the ventral ganglion of trol mutant animals. Quantitative RT-PCR analysis of the expression of the Hh response gene ptc (black bars) and the Bnl response gene pnt (grey bars) in the ventral ganglia of trol mutant normalized to controls at one hour post molt to second instar. β-actin was used to as an internal control to normalize message levels. All analyses were done in triplicate and three different concentrations to ensure samples were within linear range of amplification. Error bars indicate standard deviation.