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Figure 2 | BMC Developmental Biology

Figure 2

From: Apposition of iroquois expressing and non-expressing cells leads to cell sorting and fold formation in the Drosophilaimaginal wing disc

Figure 2

Morphology of iroDFM3clones located within the notum territory. iroDFM3cells: absence of green. (A-H) Ed apical marker, red. (E-G) TO-PRO-3 nuclear marker, blue. (A, B) Conventional and optical z section views respectively of iroDFM3clones induced 72 to 96 h AEL. Arrowheads identify the same clone. Arrow: section through the notum/hinge fold. (C, D) Optical z section views of two different and larger iroDFM3clones with the apical parts of their cells in aposed and separated configurations, respectively. (E-G)xy and two z section views, respectively, of a "W" shaped iroDFM3clone. Dashes mark the approximate plane of the indicated views. Arrowhead: wild-type cells form the more apical region of the pouch. (H, I)z section views of two early induced (48–72h AEL and 24–48h AEL) clones stained for either Ed or Actin, respectively (red). Insets in I show the two channel xy (left) and z (right) views of the clone. Only the red channel is shown in the main panels. Blue and yellow dots mark the apical and basal regions, respectively, of the cells of the disc epithelium. (J) Suggested transition from pouch-shaped to cylindrical iroDFM3clones that would occur during the development of the disc. iroDFM3cells: grey. Apical regions: red. Round cells: adepithelial cells.

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