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Figure 7 | BMC Developmental Biology

Figure 7

From: Loss of spermatogonia and wide-spread DNA methylation defects in newborn male mice deficient in DNMT3L

Figure 7

Expression of DNA methyltransferases in Dnmt3L-/- male germ cells. QRT-PCR was used to evaluate the relative expression of Dnmt genes in isolated Dnmt3L mutant 6 dpp primitive type A spermatogonia. Expression of a given Dnmt was analyzed in triplicate and normalized to 18S expression for each genotype; the normalized value was calibrated to the expression observed in Dnmt3L-/- cells. A) Illustration of Dnmt3L expression in Dnmt3L+/+, Dnmt3L+/- or Dnmt3L-/- male germ cells. Dnmt3L could not be detected in the Dnmt3L-/- sample. Only the normalized values are presented in this case, calibrating the data to the Dnmt3L-/- sample being impossible. ND, not detectable. B) Expression of Dnmt3a, Dnmt3a2, Dnmt3b and Dnmt1 in Dnmt3L+/+ (black bars), Dnmt3L+/- (grey bars) and Dnmt3L-/- (white bars) germ cells. No observable differences in DNA methyltransferase expression could be detected in Dnmt3L-deficient germ cells at 6 dpp. Results from one series of germ cells are presented as mean ± SD.

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