Figure 1

Gene targeting into the embryonic retina. (A) Schematic representation of retinal electroporation in utero. A small amount of DNA is injected into the embryo's eye through the uterine wall (left), and then electric pulses are passed using paddle electrodes. The result is the delivery of DNA to a subset of retinal cells (right). Only when the positive electrode was located on the injected eye was the electroporation successful. (B) Retinal section of an E16 embryo electroporated at E13. GFP expressing cells can be detected in the central part of the retina (arrows), surrounding the optic disc; scale bar: 200 μm. (C, D, E) Flattened whole mounts of E16 retinas electroporated at E13 after injection of different volumes of GFP-plasmid solution (0.2 μl, 0.5 μl and 1 μl respectively of a 1 μg/μl DNA solution) show the increase in the number cells targeted in the central retina (cells in the dashed circle). Scale bar: 500 μm.