Figure 6From: Sea urchin vault structure, composition, and differential localization during developmentConfocal images of sea urchin embryos at various developmental stages. The cellular distribution of MVP is revealed by staining with an affinity-purified polyclonal antiserum against the sea urchin MVP and secondary staining with Oregon Green-conjugated anti-rabbit antibody (A, D, G, J; left column). The nuclei are stained with DAPI (B, E, H, K; middle column). By merging the MVP and DNA images coincident staining is observed as cyan (C, F, I, L; right column). Note that as development proceeds the cellular localization of MVP progressively shifts from cytoplasmic to nuclear.Back to article page