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Table 2 Ranking of the reference genes in order of their expression stability per assay, decreasing from top to bottom. The reference genes chosen to calculate the normalization factor are printed in bold.

From: Selection of reference genes for quantitative real-time PCR in bovine preimplantation embryos

SDHA SDHA GAPD GAPD
Assay 1 Assay 2 Assay 3 Combined*
18S rRNA GAPD YWHAZ YWHAZ
GAPD 18S rRNA 18S rRNA 18S rRNA
YWHAZ YWHAZ SDHA SDHA
HPRT1 Histone H2A Histone H2A Histone H2A
Histone H2A HPRT1 TBP HPRT1
TBP TBP HPRT1 TBP
ACTB ACTB ACTB ACTB
  1. *After correction for run-to-run variation