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Table 2 Ranking of the reference genes in order of their expression stability per assay, decreasing from top to bottom. The reference genes chosen to calculate the normalization factor are printed in bold.

From: Selection of reference genes for quantitative real-time PCR in bovine preimplantation embryos

SDHA

SDHA

GAPD

GAPD

Assay 1

Assay 2

Assay 3

Combined*

18S rRNA

GAPD

YWHAZ

YWHAZ

GAPD

18S rRNA

18S rRNA

18S rRNA

YWHAZ

YWHAZ

SDHA

SDHA

HPRT1

Histone H2A

Histone H2A

Histone H2A

Histone H2A

HPRT1

TBP

HPRT1

TBP

TBP

HPRT1

TBP

ACTB

ACTB

ACTB

ACTB

  1. *After correction for run-to-run variation