Table 1 Information on the primers used for real-time PCR
From: Selection of reference genes for quantitative real-time PCR in bovine preimplantation embryos
Gene | Genbank Accession number | species | Sequence | Product size (bp) | Ta (°C) | % homology |
|---|---|---|---|---|---|---|
ACTB [23] | AY141970 | Cow | 5'-CCTCACGGAACGTGGTTACA-3' 5'-TCCTTGATGTCACGCACAATTT-3' | 87 | 58 | 100 % |
GAPD | XM_618013 | Cow | 5'-TTCAACGGCACAGTCAAGG-3' 5'-ACATACTCAGCACCAGCATCAC-3' | 119 | 62 | 100 % |
Histone H2A [15] | U62674 | Mouse | 5'-GTCGTGGCAAGCAAGGAG-3' 5'-GATCTCGGCCGTTAGGTACTC-3' | 182 | 60 | 82 % |
TBP [33] | NM_003194 | Human | 5'-CCTAAAGACCATTGCACTTCG-3' 5'-CTTCACTCTTGGCTCCTGTG-3' | 146 | 57 | 94 % |
HPRT1 | AF176419 | Cow | 5'-TGCTGAGGATTTGGAGAAGG-3' 5'-CAACAGGTCGGCAAAGAACT-3' | 154 | 58 | 100 % |
SDHA | NM_174178 | Cow | 5'-GCAGAACCTGATGCTTTGTG-3' 5'-CGTAGGAGAGCGTGTGCTT-3' | 185 | 60 | 100 % |
YWHAZ | BM446307 | Cow | 5'-GCATCCCACAGACTATTTCC-3' 5'-GCAAAGACAATGACAGACCA-3' | 120 | 60 | 97 % |
18S rRNA | AF176811 | Cow | 5'-AGAAACGGCTACCACATCCA-3' 5'-CACCAGACTTGCCCTCCA-3' | 169 | 62 | 100 % |