Figure 1

(A-B): Gene expression stability of the candidate reference genes analyzed by the geNorm program. (A) Average expression stability values (M) of the control genes over the 3 assays together, plotted from least stable (left) to most stable (right). (B) Pairwise variation analysis over the 3 assays together between the normalization factors NF_n and NF_n+1, to determine the optimal number of control genes for normalization. The higher V_4/5 and V_7/8 values are due to the inclusion of a relative unstable gene and are in accordance with the average expression stability M.