Figure 5

Positioning of functionally similar olfactory neurons within the antennal nerve. Dissected brains of Or22a-Gal4;UAS-N-SybGFP (A) and Or47b-Gal4;UAS- N-SybGFP (B,C) were stained with antibodies against GFP. The antennal nerves (AN) are marked with white dots and the boundaries of the olfactory lobe (OL) by yellow dotted lines. Or22a-neurons travel in different regions of the antennal nerve (thin arrows) to enter the DM2 glomerulus in two bundles (arrowheads). Similarly Or47b axons traverse the nerve to reach VA1 glomerulus and also cross the midline (marked in *) to the contralateral lobe. The image in C is magnified (scale bar = 30 μm) and shows that neurons expressing the same Or gene do not group together within the lobe. Scale bar = 40 μm in A, B.