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Figure 2 | BMC Developmental Biology

Figure 2

From: Comparison of the gene expression profile of undifferentiated human embryonic stem cell lines and differentiating embryoid bodies

Figure 2

RT-PCR of some marker genes in pooled samples of ES and EB RNA. RT-PCR analysis of marker genes in Pooled ES and pooled EB cell lines. Total RNA derived from both the ES and EB cell lines (WiCell lines) were subjected to RT-PCR analysis as previously described (34). G3PDH mRNA amplified from these samples served as an internal control. The primers used are listed in supplementary table (Table 7S). The thermocycler conditions used for amplification were 94°C, 4 minutes hot start,94°C, 30 sec; 60°C, 30 sec and 72°C, 1 minute. Ten microliter amplification products were resolved in 2% agarose gel, stained with ethidium bromide (EtBr), and visualized in a transilluminator and photographed.

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