Figure 2

Quantitation of CFTR expression following infection with AdCMVAScftr. Fetuses were treated at 16 days gestation with either AdCMVlacZ (control) or AdCMVAScftr and lungs harvest at 24 and 72 hours post-gene transfer. Lungs from 5 animals were cryo-sectioned and CFTR visualized by immunohistochemistry. Image analysis on the deconvoluting microscope was performed and the results standardized for the number of cells by nuclear staining with DAPI