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Figure 5 | BMC Developmental Biology

Figure 5

From: Expressionof Drosophila FOXO regulates growth and can phenocopy starvation

Figure 5

dFOXO inactivation is essential for dAkt, but not dPI3K,mediated increases in cell size. Ommatidia area was measuredas a means to determine the effect of FOXO overexpression on cellsize. Expression of dFOXO (bar 2), mFoxo1 (bar 3), and mFoxo1-AA(bar 4) under the control of GMR-Gal4 causes a significantdecrease in ommatidia area when compared to the expression of Gal4alone (bar 1). In addition, GMR-Gal4 was used to drivethe expression of dPI3K (bars 5–8), and UAS-dAkt (bars9–12), either alone (grey bars), or in the presence of UAS-dFOXO (redbars), UAS-mFoxo1 (light green bars), or UAS-mFoxo1-AA (darkgreen bars). Two sided t-tests were preformed to determine statisticalsignificance (p = 0.001). Genotypes are: (1) w; GMR-Gal4/+,(2) w; GMR-Gal4/+; UAS-dFOXO/+, (3) w; GMR-Gal4, UAS-mFoxo1/+,(4) w, UAS-mFoxo1-AA/w; GMR-Gal4/+, (5) w; UAS-dPI3K/GMR-Gal4,(6) w; UAS-dPI3K/ GMR-Gal4; UAS-dFOXO/+, (7) w; GMR-Gal4,UAS-mFoxo1/UAS-dPI3K, (8) w, UAS-mFoxo1-AA/w; GMR-Gal4/UAS-dPI3K,(9) w; UAS-dAkt/GMR-Gal4, (10) w; UAS-dAkt/GMR-Gal4;UAS-dFOXO/+ (11) w; GMR-Gal4, UAS-mFoxo1/UAS-dAkt (12) w,UAS-mFoxo1-AA/w; GMR-Gal4/UAS-dAkt.

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