Figure 3

dFOXO reduces growth through alterations in cell sizeand cell number (A) Expression of UAS-dFOXO in the thirdlarval instar produces small flies (left) when compared to controls(right). w; hsGal4/CyO flies were crossed to w; UAS-dFOXO/UAS-dFOXO fliesand the progeny were heat shocked at 37°C for 4 hours during theearly third instar. (B) Flies of the genotype w; hsGal4/+; UAS-dFOXO/+ (left)were smaller than w; hsGal4/+ (right) flies when raisedat 29°C. (C) The wings of w; hsGal4/+; UAS-dFOXO/+ fliesraised at 29°C were smaller than control wings (scale bar = 1 mm).(D) Flies expressing dFOXO (red bars) also showed a significant reductionin body weight, wing area, cell number, and cell size when comparedto control flies (grey bars) (p = 0.005). (E) Flies expressing dFOXOhad smaller eyes than control flies (scale bar = 150 μm), and (F)their eyes were reduced in both the number of ommatidia and thearea of the ommatidia (red bars) when compared to controls (greybars). Genotypes are; (A-left, B-left, C-top, D-red bars, E-left,F-red bars) w; hsGal4/+; UAS-dFOXO/+, (A-right, B-right,C-bottom, D-grey bars, E-right, F-grey bars). w; hs-Gal4/+.