Figure 1

The arista phenotypes of frizzled pathway mutants. Panels A – D show adult aristae from A – wild type, B – fz, C – fy, and D – mwh flies. The arrows in panels C and D point to split and/or multipled laterals. Panels E – M are confocal images of Alexa 588 phalloidin (Molecular Probes) stained aristae. Panel E is wild type (30 hr apf (after prepupae formation)(arrowheads point to laterals forming near the distal edge of the cell), F – fz (30 hr apf) (arrows point to laterals forming at a central location in the cell), G – dsh (30 hr apf)(arrows as in F), H – mwh (31 hr apf)(arrow points to a cell that is forming multiple independent laterals), I – fy (28 hr apf) (arrows point to regions of actin accumulation), J – fy (30 hr apf) (arrow point to cells with multiple independent laterals), K – fy (36 hr apf) (arrow heads point to branched laterals), L – mwh (36 hr apf) (arrow points to a cell with multiple independent laterals and the arrowhead points to a branched lateral) and M – mwh 42 hr apf. Pupae were fixed and stained using standard procedures [8].