Figure 3
From: Ecdysteroids and oocyte development in the black fly Simulium vittatum
Vitellogenin accumulation in Simulium vittatum ovaries after eclosion. Ovaries were homogenized in SDS sample buffer. Proteins were separated on SDS-PAGE and stained with Coomassie blue. The intensity of the stain in the 200 kDa protein band was determined by densitometry. Data are reported as the mean ± standard deviation relative to the density of the 200 kDa band at 12 hours to correct for differences in staining intensity between gels. The number of band measurements used to calculate the mean is shown next to the data points.