Figure 2

Phase contrast photomicrographs of human amniotic fluid cultured cells and spontaneous differentiation of clone cells into cells of three embryonic germ layers. (A, B) hAFCs formed clones (white arrows) and attached cells (black arrows) after 5–7 days in culture. (C) Clone cells yielded embryoid bodies (EBs) (black arrow). (D) Scatter plots and bar graph for flow cytometry analysis of OCT4 and CD117 expression in attached cells (hAFC) and clones (hAFC-clone). The percentage of Oct4+/CD117+ expressing cells was higher (7.8%) in clone than attached cells (1.81%; ***P < 0.001) (** P < 0.01, * P < 0.05). (E) Embryoid bodies spontaneously differentiated into cells stained with Sox17, muscle actin and Nestin respectively. Magnification, 100x (A), 200x (B, C, E).