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Figure 9 | BMC Developmental Biology

Figure 9

From: Changes in sub-cellular localisation of trophoblast and inner cell mass specific transcription factors during bovine preimplantation development

Figure 9

CDX2 displays nuclear localisation and labels metaphase chromosomes. (a) presents 2 consecutive optical sections through an early bovine embryo double labelled for CDX2 and for histone lysine methylation H3K9me3. White arrow heads point to a typical heterochromatin distribution pattern at the periphery of the interphase nucleus. Green arrow heads indicate CDX2 and H3K9me3 colocalisation. Dashed circle in (a) highlights the metaphase plate. CDX2 signal overlays the chromosomes and colocalises with condensed, transcriptionally silent chromatin as indicated by H3K9me3 immunostaining enlarged in the right panel. (b) presents an optical scan through middle of late morula embryo (beginnings of cavity formation may be visible in top section of the embryo). CDX2 displays nuclear localisation and also labels the metaphase chromosomes (marked by the dashed white circle, the area is enlarged in the boxed panel). (c) HBl (9dpi) immunostained for CDX2. Dashed white circle highlights the metaphase plate where, the encircled area is enlarged in the boxed panel where the CDX2 signal colocalisation with metaphase chromosomes is visible. DAPI marks chromatin. Confocal sections were taken every 3 μm.

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