Figure 7From: Changes in sub-cellular localisation of trophoblast and inner cell mass specific transcription factors during bovine preimplantation developmentOCT4 and NANOG distribution pattern in bovine hatched blastocysts (9dpi). The top panel (a) of images shows a 3-D reconstruction of confocal sections taken through bovine HBL. Dashed line encircles the ICM. The bottom panel (b) represents a single optical section through the ICM of the same embryo. OCT4 and NANOG localise in the ICM, but a certain degree of signal segregation was observed. Cells indicated with red arrowheads express NANOG and cells indicated with white arrowheads co-express both proteins. DAPI marks chromatin. Confocal sections were taken every 3 μm.Back to article page