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Figure 5 | BMC Developmental Biology

Figure 5

From: Changes in sub-cellular localisation of trophoblast and inner cell mass specific transcription factors during bovine preimplantation development

Figure 5

Distribution of OCT4 and NANOG in the early stages of bovine development. Immunofluorescent detection of OCT4 (a) and NANOG (b) followed through the early stages of bovine development. In 5-cell embryos OCT4 (a) localised at the apical surface of the blastomeres, and from the 8–16 cell stage weak signal was detected in cell nuclei and mostly absent in the cytoplasm. NANOG (b) immunostaining was for the first time detected at the morula stage exhibiting a specific cytoplasmic localisation with characteristic rings around the nuclear periphery (indicated by arrow heads). From the early stages of blastocyst formation OCT4 and NANOG signals were detected in the nuclei of the ICM cells. Confocal sections were taken every 3 μm. Chromatin was labelled with DAPI.

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