Figure 2

Visualizing movement kinetics in the postnatal mouse retina. (A) A time series demonstrating changes in morphology and position of transfected retinal cells. Postnatal day 0 mouse retinas were co-electroporated with a nuclear-localized GFP, CMV-Cre and pCALNL-dsRed. Image acquisition began at 24h post-transfection with a 3h per frame temporal resolution. Open arrowheads indicate an emerging eGFP nucleus in the differentiating iNBL. Bracket in the final frame indicates the range of movement by a nucleus within a GFP/dsRed co-localized cell. NBL – neuroblastic layer; iNBL – inner neuroblastic layer. (B) A selection of images from a time series demonstrating mitotic events (yellow arrowheads) by H2B-GFP expressing nuclei. (C). Cell tracking histograms of nuclear movements for H2B-GFP (i.) and knockdown (ii.) retinas. Blue lines and arrows represent the largest range of movement by a single nucleus, whereas green lines and arrows represent the smallest range. Yellow arrowheads indicate mitotic events, and correspond to yellow arrowheads in (B). (iii) is a representative live frame of H2B-GFP distribution across the apical NBL margin (aNBL) and iNBL. (D) Comparison of H2B-GFP control nuclear tracing data with knockdown nuclei.