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Figure 4 | BMC Developmental Biology

Figure 4

From: The C. elegans gene pan-1encodes novel transmembrane and cytoplasmic leucine-rich repeat proteins and promotes molting and the larva to adult transition

Figure 4

Hypodermal defects of arrested pan-1(RNAi) animals. Epifluorescence micrographs of ajm-1::GFP (A, B) and col-19::GFP (C, D) in control (A, C) and pan-1 RNAi (B, D) animals. For (A) and (B), the areas marked by the blue box are shown as enlarged DIC images (A’ and B’). Animals subjected to pan-1 RNAi do not synthesize alae (arrows in A’) in areas of normal seam fusion. The arrows in (B) represent areas of abnormal seam morphology including lack of fusion and abnormal morphology (arrow marked with an asterisk). (C) and (D) col-19::GFP is not expressed in arrested pan-1(RNAi) animals. (E) Quantification of seam phenotypes in control (red bar) and pan-1 (blue bar) RNAi animals. The stippling indicates the proportion of animals exhibiting completely normal seam morphology. N = 41 for pan-1 RNAi; N = 20 for control RNAi. Scale bars = 10 μm.

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BMC Developmental Biology

ISSN: 1471-213X

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